Perbandingan sitotoksisitas tiga jenis algyrogel terhadap sel fibroblas (Citotoxicity comparation of three types of algyrogel on fibroblast cells)

Stefany Elan Saktiyawardani, Hardono Jaya Lauson, Anugerah Pekerti Astamurtiningrum, Mahadna Aulia Rahmah, Pramana Pananja Putra, Juni Handajani
2014 Dental Journal: Majalah Kedokteran Gigi  
The prevalence of osteoporosis and bone defects in the world and Indonesia increase each year. Algyrogel is a alginate-based hydrogel material recently very popular used as a bone substitute through injectable bone substitute (IBs) method. One of advantages algyrogel is biocompatibility to the body tissues. some natural-based polymer (alginate, chitosan, dan hyaluronat) have different biocompatibility. Purpose: The study was aimed to determine the cytotoxicity of three types algyrogel against
more » ... algyrogel against fibroblast cells. Methods: This study used three types algyrogel, there were algyrogel containing alginate (Alg), a mixture of alginate with chitosan (Alg/Ch), and a mixture of alginate with hyaluronic acid (Alg/Ha). Each type algyrogel was exposed in the Vero cell line of fibroblast with volume 2 μL; 4 μL; 6 μL; 8 μL; 10 μL; 12 μL; 14 μL; 16 μL; 18 μL; and 20 μL respectively. The cytotoxicity performed by MTT method to determine the percentage of cell death. The optical density was measured by an ELIsA plate reader then data was analyzed using ANOVA and probit. results: The percentage of cell death in all groups of algyrogel have below 30%. LC50 value at Alg was 651.017 mg/mL (21.56%), Alg/Ch was 280.478 mg/mL (20.91%), and Alg/Ha was 1054.094 mg/ mL (17.2%). Conclusion: Alg/Ha has the lowest cytotoxicity on fibroblast cells and may have potential as an osteoconductor-synthetic bone product.
doi:10.20473/j.djmkg.v47.i3.p130-134 fatcat:xbkc7dimnngplmldnxyjegrzhy