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Derepression of Anthranilate Synthase in Purified Minicells of Escherichia coli Containing the Col-trp Plasmid
1973
Journal of Bacteriology
Purified minicells of Escherichia coli K-12 containing the plasmid Col-trp+ or Col-trpA2 could be derepressed for the synthesis of anthranilate synthase, the first enzyme encoded in the trp operon. Non-plasmid-containing, deoxyribonucleic acid-deficient minicells could not be derepressed. Derepressed enzyme synthesis was initiated by L-tryptophan starvation. The kinetics of derepression were studied with minicells containing the Col-trpA2 plasmid. The derepression curves were biphasic with a
doi:10.1128/jb.115.2.615-622.1973
fatcat:6bji36w64zgyhfizqt23pu4myi