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Aseptically, shoot bud and undifferentiated cultures of globe artichoke were successfully stored for 12 months at 5ºC in the dark. Under these conditions, high percentage of cultures remained viable without perceptable serious signs of senenscence. Although the rates of regrowth slightly decreased, the recovery percentages were enough to obtain high frequencies of healthy globe artichoke (= Cynara scolymus L.). The storage at cold and dark conditions was strongly effective compared with storagedoi:10.3329/ptcb.v17i1.1115 fatcat:d3mmkv2a25dvhfbaehdjm4swwu