In vitro Preservation of Globe Artichoke Germplasm

SA Bekheet
2008 Plant Tissue Culture and Biotechnology  
Aseptically, shoot bud and undifferentiated cultures of globe artichoke were successfully stored for 12 months at 5ºC in the dark. Under these conditions, high percentage of cultures remained viable without perceptable serious signs of senenscence. Although the rates of regrowth slightly decreased, the recovery percentages were enough to obtain high frequencies of healthy globe artichoke (= Cynara scolymus L.). The storage at cold and dark conditions was strongly effective compared with storage
more » ... in the medium under osmotic stress. Also, the differentiated cultures registered a higher viability compared to undifferentiated cultures. RAPD analysis suggests that plantlets derived from the preserved cultures were identical to those derived from nonstored differentiated tissues culrtures. However, undifferentiated (callus) cultures showed relatively less genetic variation.
doi:10.3329/ptcb.v17i1.1115 fatcat:d3mmkv2a25dvhfbaehdjm4swwu