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Fluorescent labeling is the prevailing imaging technique in cell biological research. When statistical investigations on a large number of cells are involved, experimental study is required for both low magnification to get a reliable statistical population and high contrast to achieve accurate diagnosis on the nature of the cells' perturbation. As microscope objectives of low magnification generally yield low collection efficiency, such studies are limited by the fluorescence signal weakness.doi:10.1155/2014/921489 fatcat:c34ivpt3gncunnntgqq2p5qxda