In vivo [3H]Spiperone Binding: Evidence for Accumulation in Corpus Striatum by Agonist-Mediated Receptor Internalization

Diane C. Chugani, Robert F. Ackermann, Michael E. Phelps
1988 Journal of Cerebral Blood Flow and Metabolism  
The processes of receptor internalization and recycling have been well-documented for receptors for hormones, growth factors, lysosomal enzymes, and cel lular substrates. Evidence also exists that these pro cesses also occur for f)-adrenergic, muscarinic cholin ergic, and a-opiate receptors in frog erythrocytes or cul tured nervous tissue. In this study, evidence is presented that agonist-mediated receptor internalization and recy cling occurs at the dopamine receptor in rat corpus striatum.
more » ... st, the in vivo binding of the dopamine an tagonist [3H]spiperone was increased by both electrical stimulation and pharmacologically induced increases of dopamine release. Conversely, depletion of dopamine with reserpine decreased in vivo [3H]spiperone binding, but the same reserpine treatment did not alter its in vitro binding. Second, the rate of dissociation of [3H]spiperone from microsomal membranes prepared from rat striatum
doi:10.1038/jcbfm.1988.64 pmid:2966803 fatcat:urxmol2u2fbwjfxlabzzxdwaoi