Generation of Monoclonal Antibodies against a Feline CD Antigen (CD4) Expressed by a Recombinant Baculovirus

Masayuki SHIMOJIMA, Shigeru MORIKAWA, Ken MAEDA, Yukinobu TOHYA, Takayuki MIYAZAWA, Takeshi MIKAMI
1997 Journal of Veterinary Medical Science  
We attempted to establish a system to generate monoclonal antibodies (mAbs) recognizing CD antigens on lymphocytes of domestic animals using the expressed CD antigens by a baculovirus expression system. For this purpose, we selected feline CD4 (fCD4) antigen and expressed it in an insect cell line (Sf9 cells). To obtain mAbs, BALB/c mice were immunized with feline peripheral blood mononuclear cells (fPBMCs) or Sf9 cells expressing the fCD4 (Sf-fCD4 cells), and then hybridomas secreting
more » ... secreting antibodies were screened by indirect immunofluorescence assay against the opposite antigens of Sf-fCD4 cells or fPBMCs, respectively. Five mAbs recognizing the fCD4 were obtained in total. The system established here might be useful to obtain mAbs recognizing CD antigens of domestic animals. -KEY WORDS: baculovirus, feline CD4 antigen, monoclonal antibody.
doi:10.1292/jvms.59.467 pmid:9234223 fatcat:3m2kw63ajre5zlqme7j764xp4e