Labeling Cells with Silver/Dendrimer Nanocomposites

Wojciech Lesniak, Xiangyang Shi, Anna Bielinska, Katarzyna Janczak, Kai Sun, James R. Baker, Lajos P. Balogh
2004 Materials Research Society Symposium Proceedings  
We have developed water-soluble, biocompatible, fluorescent, and photostable silver/dendrimer nanocomposites that have a potential to be used for in vitro cell labeling. A PAMAME5.NH 2 dendrimer was used as a template to prepare first a silver-dendrimer complex in an aqueous solution at biologic pH=7.4. Conversion into nanocomposite was achieved by irradiating the solution of the [(Ag+) 25 -PAMAM E5.NH 2 ] complex by UV light to reduce the bound Ag + to zero-valent Ag o atoms, which were
more » ... , which were simultaneously trapped in the dendrimer network. Results indicate that the {(Ag°) 25 -PAMAME5.NH 2 } silver/dendrimer nanocomposite forms positively charged single particles of 4-5 nm under the experimental conditions used. The dendrimer nanocomposite proved to be fluorescent. Toxicity testing of {(Ag°)z 5 -PAMAM_E5.NH 2 nanocomposite revealed a behavior similar to the template dendrimer. Intracellular internalization of the silver nanocomposite and cell labeling capabilities was confirmed by confocal microscopy.
doi:10.1557/proc-845-aa5.37 fatcat:7s6rrcood5gejc7cblgbc44voy