The purpose of this study was to find a method of determining laser models and intra vaginal progesterone sponge in Bali Cattle. An assessment was carried out at Sapi Loka Grati, Pasuruan, Indonesia. The parameters observed included the symptoms of estrus, response of estrus, and the level of pregnancy. Twenty Balinese cows were divided at random in two groups. P1 treated with laser puncture at a certain point and P2 treated with 1.5 g of progesterone + 10 mg of estradiol benzoate intra vaginal

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... sponge. The diagnosis of subsequent pregnancies was done by ultrasonographic examination on day 40 th after artificial insemination (AI). The laser used in this study was a soft laser with power supply specifications as follows: 50 Hz, 220 Volts, 50 Watts, ranging from 0.2 Joules to 0.5 Joules. These lasers include semiconductor lasers and laser probes. Three acupuncture points were used (GV 4, BL-22, and GV-2). Furthermore, the intra vaginal progesterone sponge used was made from silicone plastic similar to the drugs containing medroxyprogesterone acetate. Data testing was done by using the T-test. Results demonstrated that the P1 responses of estrus appeared on day 3 rd , while P2 on day 9 th . There was no significant difference (p >0.05) in the accumulation of estrus and pregnancy. In conclusion, estrus synchronization and pregnancy rate could be efficiently achieved in Bali cattle using either laser puncture or intra vaginal progesterone sponge. However, laser puncture treatment induced more spread estrus with higher pregnancy rate.