Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. ciliata (Commelinaceae)

Koki R. Katsuhara, Naoyuki Nakahama, Taketo Komura, Masaya Kato, Yuko Miyazaki, Yuji Isagi, Motomi Ito, Atushi Ushimaru
2019 Genes & Genetic Systems  
Commelina communis f. ciliata (Commelinaceae), a newly distinguished taxon, is an annual andromonoecious herb exhibiting a mixed mating system, the details of which remain unclear. We developed microsatellite markers for use in exploring the evolution of andromonoecy and mixed mating in the species. Fifteen microsatellite loci were developed using next-generation sequencing. The primer sets were used to evaluate 65 C. communis f. ciliata individuals from three populations in Japan; we found
more » ... Japan; we found 1-13 alleles per locus and the expected heterozygosity ranged from 0.00 to 0.76. The markers are potentially useful to examine intra- and interspecies genetic structure and the mixed mating strategy of Commelina species via paternity analysis.
doi:10.1266/ggs.18-00058 pmid:31257310 fatcat:6tm6f6jsnffidgzpozlg4yot6e