Survey on indirect optical manipulation of cells, nucleic acids, and motor proteins

Ashis Gopal Banerjee, Sagar Chowdhury, Wolfgang Losert, Satyandra K. Gupta
2011 Journal of Biomedical Optics  
Optical tweezers have emerged as one of the most promising non-contact manipulation techniques for biological objects. Instead of direct laser exposure, more often than not, optically-trapped beads are attached to the ends or boundaries of the objects for translation, rotation, and stretching. This is referred to as indirect optical manipulation. In this paper, we utilize the concept of robotic gripping to explain the different experimental set-ups which are commonly used for indirect
more » ... on of cells, nucleic acids, and motor proteins. We also give an overview of the kinds of biological insights provided by this technique. We conclude by highlighting the trends across the experimental studies, and discussing challenges and promising directions in this domain of active current research.
doi:10.1117/1.3579200 pmid:21639562 fatcat:vyaavueasrcgdgxfrrmif2o6wa