Wilms' tumor gene 17AA(-)/KTS(-)- reduced ability of cancer cell adhesion to fibronectin substratum

Tanyarat Jomgeow, Singkome Tima
2009 Journal of Associated Medical Sciences  
Wilms'tumor gene (WT1) 17AA(-)/KTS(-) isoform alters morphological changes, motility and invasion of cancer cells that may involve in cell attachment to extracellular matrix (ECM) and its receptor integrin subunits. The objective of this study is to determine effect of WT1 17AA(-)/KTS(-) cell adhesion on each type of ECM coating substratum in ovarian cancer and fibrosarcoma cell line. Constitutively expression of WT1 17AA(-)/KTS(-) in both cell lines were determined cell adhesion assay to
more » ... sion assay to Fibronectin, Collagen I or Laminin substratum compared to mock control cells. We found that WT1 17AA(-)/KTS(-) reduced the ability of cancer cells to Fibronectin substratum. This data suggested that 17AA(-)/KTS(-) may has function in cancer cells adhesion mechanism and may has highly possible to involve the function of Fibronectin substratum binding-integrin subunits that related to cancer cells morphological changes, motility and invasion. Bull Chiang Mai Assoc Med Sci 2009; 42: 233-241.
doaj:bdb4e0f5db0a448cbf437c6175a1d6e8 fatcat:lxksvja7qngclcz6qok3gayjee