The transcription activator c-Myb is expressed at high levels in immature thymocytes and during T-cell activation and may be a regulator of T-cell differentiation. To investigate the role of c-Myb in T-cell development, we generated transgenic mice in which two dominant interfering Myb alleles, one a competitive inhibitor of DNA binding, and the other, an active repressor comprising the Myb DNA-binding domain linked to the Drosophila Engrailed transcription repressor domain, were expressed from

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... early times onward in T cells. Both alleles partially blocked thymopoiesis and inhibited proliferation of mature T cells. The Myb-En chimera was the more efficient repressor and might serve as an archetype for the manufacture of other dominant interfering transcription factor alleles. [The c-myb proto-oncogene encodes a DNA-binding tranmain fused to the repressor domain of the Drosophila Engrailed (En) transcription factor. This alanine-rich region is a strong active repressor of transcription (]aynes and O'Farrell 1991; Han and Manley 1993}, and is fur-770 GENES & DEVELOPMENT 8:770-782 -Published by genesdev.cshlp.org Downloaded from 10.1101/gad.8.7.770 Access the most recent version at doi: 8: 1994, Genes Dev. P Badiani, P Corbella, D Kioussis, et al. development. Dominant interfering alleles define a role for c-Myb in T-cell References http://genesdev.cshlp.org/content/8/7/770.full.html#ref-list-1