Original Article Cheonwangbosimdan, a traditional herbal formula, inhibits inflammatory responses through inactivation of NF-κB and induction of heme oxygenase-1 in RAW264.7 murine macrophages

Ohn-Soon Kim, Yeji Kim, Sae-Rom Yoo, Mee-Young Lee, Hyeun-Kyoo Shin, Soo-Jin Jeong
2016 Int J Clin Exp Med   unpublished
Cheonwangbosimdan (CWBSD) is a traditional herbal formula composing 15 medicinal herbs that used for treating mental disorders. In the present study, we investigated inhibitory effects of CWBSD using RAW264.7 murine macrophage cell line. We found that CWBSD induced heme oxygenase-1 (HO-1) expression in a dose-dependent manner with no cytotoxicity. CWBSD significantly reduced production of pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-α), but not interleukin-6 in lipopolysaccharide
more » ... lipopolysaccharide (LPS)-stimulated RAW264.7 cells. CWBSD also significantly inhibited LPS-induced production of pro-inflammatory mediators such as nitric oxide (NO) and prostaglandin E 2 (PGE 2). In addition, CWBSD suppressed phosphorylation of p38 mitogen-activated protein kinase (MAPK) and c-jun N-terminal kinase (JNK) as well as activation of nuclear factor kappa B (NF-κB) in LPS-treated macrophages. Overall, our data provide a foundation for further insight into the molecular mechanisms responsible for the anti-inflammatory activity of CWBSD in chronic inflammatory diseases.