Transcriptional Analysis of Stearoyl-Acyl Carrier Protein Desaturase Genes from Olive (Olea europaea) in Relation to the Oleic Acid Content of the Virgin Olive Oil [component]

1 The specific contribution of different stearoyl-ACP desaturase (SAD) genes to the oleic 2 acid content in olive (Olea europaea) fruit has been studied. Towards that end, we 3 isolated three distinct cDNA clones encoding three SAD isoforms from olive (cv. 4 Picual), as revealed by sequence analysis. The expression levels of olive SAD genes 5 were determined in different tissues from Picual and Arbequina cultivars, including 6 developing mesocarp and seed, together with the unsaturated fatty
more » ... nsaturated fatty acid content. Lipid 7 and gene expression analysis indicate that OeSAD2 seems to be the main gene 8 contributing to the oleic acid content of the olive fruit and, therefore, of the virgin olive 9 oil. This conclusion was confirmed when the study was extended to Hojiblanca, Picudo 10 and Manzanilla cultivars. Furthermore, our data indicate that the olive microsomal 11 oleate desaturase gene OeFAD2-2, but not OeSAD2, is responsible for the linoleic acid 12 content in the virgin olive oil. 13 14
doi:10.1021/acs.jafc.6b02963.s001 fatcat:mfc55efyxng5deswd3vebkivw4