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This study was carried out to study the effect of trace metal addition for hydrogen production The results show that this bacterial need a selected nutrient with selected concentration. The optimum parameters for cultivation were at initial pH of 7.0 and temperature of 30°C. Trace metal addition to the new medium showed that only iron enhanced the H 2 yield. The results showed that 25 mgL −1 FeSO 4 ⋅7H 2 O enhanced the hydrogen yield from 391 mLg −1 to 408 mLg −1 glucose utilized with biomassdoi:10.3844/ojbsci.2008.1.9 fatcat:saw666fz5bdb3mrl4naeiedwl4