Pre-harvest bagging or post-harvest ethylene treatments on lemons are commonly applied to change the surface color from green to favorable yellow. In this study, the differential mechanisms of the pigment metabolism by the two treatments were investigated by pigments contents and related genetic expression. The results showed that both treatments reduced the number of chloroplasts and the content of chlorophyll. The differential expression of PSY1 and PSY2 were observed, causing the different

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... cumulation of the main carotenoid phytoene content. The differential expression of NYC resulted in altered contents of chlorophyll a and chlorophyll b, and further led to the difference in a* value. More interestingly, the degradation of chlorophyll uncovered the color of carotenoids, leading to the color changed from green to yellow. PeerJ reviewing PDF | (Manuscript to be reviewed 1 Exploring the differential stages of the pigment metabolism by pre-harvest bagging and post-2 harvest ethylene de-greening of Eureka lemon peel 3 4 Abstract: Pre-harvest bagging or post-harvest ethylene treatments on lemons are commonly 5 applied to change the surface color from green to favorable yellow. In this study, the differential 6 mechanisms of the pigment metabolism by the two treatments were investigated by pigments 7 contents and related genetic expression. The results showed that both treatments reduced the 8 number of chloroplasts and the content of chlorophyll. The differential expression of PSY1 and 9 PSY2 were observed, causing the different accumulation of the main carotenoid phytoene 10 content. The differential expression of NYC resulted in altered contents of chlorophyll a and 11 chlorophyll b, and further led to the difference in a* value. More interestingly, the degradation of 12 chlorophyll uncovered the color of carotenoids, leading to the color changed from green to 13 yellow. 14