Purification and Crystallization of Coxsackie Virus

C. F. T. MATTERN, H. G. DUBUY
1956 Science  
Fig. 2 . Bacillus megaterium was grown in "M-9" salt-glucose medium at 34" C. The period of chilling was 45 minutes at g0C. Two hundred nuclei were counted, and the phases were identified for each test interval. Deoxyribose was identified for each test interval with Dische reagent. Purines and pyrimidines (P&P) were determined by absorption at 260 mp in a Beckman spectrophotorneter. Cells were counted in a Petroff-Hausser counting chamber. that may not be the same for E. coli. In both E. coli
more » ... d B. megaterium, there is evidence suggesting that there is a fall in the ribose moiety at the same time that there is a rise in the deoxyribose moiety. This in turn suggests that there is a conversion of ribose to deoxyribose in the synthesis of deoxyribose nucleic acid (2). Details of the methods, results, and possible interpretations of these findings will be presented in other reports (3).
doi:10.1126/science.123.3206.1037 pmid:13324144 fatcat:tsyj7dkvfvgwxo7qf2efmb3ii4