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Enhancing CRISPR deletion via pharmacological delay of DNA-PKcs
2021
CRISPR-Cas9 deletion (CRISPR-del) is the leading approach for eliminating DNA from mammalian cells and underpins a variety of genome-editing applications. Target DNA, defined by a pair of double-strand breaks (DSBs), is removed during nonhomologous end-joining (NHEJ). However, the low efficiency of CRISPR-del results in laborious experiments and false-negative results. By using an endogenous reporter system, we show that repression of the DNA-dependent protein kinase catalytic subunit
doi:10.48350/152273
fatcat:etk4kgw7wncx7e6lzpa4ttpiwu