Organ-specific distribution of AP-1 in AP-1 luciferase transgenic mice during the maturation process

Shu Ping Zhong, Wei-Ya Ma, James A. Quealy, Yiguo Zhang, Zigang Dong
2001 American Journal of Physiology. Regulatory Integrative and Comparative Physiology  
Organ-specific distribution of AP-1 in AP-1 luciferase transgenic mice during the maturation process. Am J Physiol Regulatory Integrative Comp Physiol 280: R376-R381, 2001.-Activator protein-1 (AP-1), a dimeric complex consisting of proteins encoded by the jun and fos gene families, is a transcription factor induced by a variety of signals including those eliciting proliferation, differentiation, and neoplastic transformation. Although AP-1 has been widely studied in the last decade,
more » ... decade, physiological levels of AP-1 in different tissues are unclear. In the present study, we analyzed AP-1 activity in several organs (liver, kidney, brain, lung, spleen, heart, skin) of AP-1-luciferase transgenic mice of various ages. Results of these studies indicate that the level of AP-1 in young mice is much higher than that in older mice, and, second, that the skin contains considerably higher levels of AP-1 than other organs. The level of phosphorylated extracellular signal-regulated protein kinase (ERK) in skin was higher in 1-and 2-day-old mice than in mice of other ages. In addition, phosphorylated p38 kinase was high in 2-day-old and 1-wk-old mice, but phosphorylated c-Jun NH 2 -terminal kinase was not detected at any age. AP-1 activity and level of phosphorylated ERKs declined with maturation. These results imply that AP-1 activity mediated through an ERKs-dependent pathway may be involved in skin development. skin; extracellular signal-regulated protein kinase; p38; c-Jun NH 2 -terminal kinase; activator protein-1
doi:10.1152/ajpregu.2001.280.2.r376 pmid:11208564 fatcat:cbm7doheevbdtbohd4dtwtv3cu