Redox and Functional Analysis of the Rieske Ferredoxin Component of the Toluene 4-Monooxygenase†

Nathaniel L. Elsen, Luke A. Moe, Lea A. McMartin, Brian G. Fox
2007 Biochemistry  
Toluene 4-monooxygenase catalyzes the NADH-and O 2 -dependent hydroxylation of toluene to form p-cresol. The four-protein complex consists of a diiron hydroxylase, an oxidoreductase, a catalytic effector protein, and a Rieske-type ferredoxin (T4moC). Phylogenetic analysis suggests that T4moC is part of a clade specialized for reaction with diiron hydroxylases, possibly reflected in the conservation of W69, whose indole side chain makes close contacts with a bridging sulfide. In order to further
more » ... investigate the possible origins of this specialization, T4moC, mutated variants of T4moC, and three other purified ferredoxins (the Thermus Rieske protein, the Burkeholderia cepacia Rieske-type biphenyl dioxygenase ferredoxin BphF, and the Ralstonia pickettii PK01 toluene monooxygenase TbuB, the Rieske-type ferredoxin from another diiron monooxygenase complex) were studied by redox potential measurements and their ability to complement the catalytic function of the reconstituted toluene 4-monooxygenase complex. A saturation mutagenesis of T4moC W69 indicates that an aromatic residue may modulate the redox potential and is also necessary for activity and/or stability. The redox potential of T4moC was determined to be -173 mV, W69F T4moC was -139 mV, and TbuB was -150 mV. For comparison, BphF had a redox potential of -157 mV [Couture et al. (2001) Biochemistry 40, 84-92]. Of these ferredoxins, all except BphF were able to provide catalytic activity. Given the range in redox potentials observed in the active ferredoxins, shape and electrostatics are strongly implicated in the catalytic specialization. Mutagenesis of other T4moC surface residues gave further insight into possible origins of catalytic specialization. Thus R65A T4moC gave an alteration in apparent K M only, while D82A/D83A T4moC gave alterations in both apparent k cat and K M . Since the different catalytic results were obtained by mutagenesis of residues lying on different sides of the protein adjacent to the [2Fe-2S] cluster, the results suggest that two different faces of T4moC may be involved in protein-protein interactions during catalysis. . 1 Abbreviations: T4MO, four-protein toluene 4-monooxygenase complex from Pseudomonas mendocina KR1; T4moH, hydroxylase component of T4MO; T4moC, Rieske-type [2Fe-2S] component of T4MO; TbuB, Rieske-type ferredoxin of the toluene monooxygenase complex from Ralstonia pickettii PK01; BphF, Rieske-type ferredoxin of the biphenyl dioxygenase complex from Burkeholderia cepacia strain LB400; Tt, Rieske protein from Thermus thermophilus. 976 Biochemistry 2007, 46, 976-986 10.
doi:10.1021/bi0616145 pmid:17240981 fatcat:tm6y62h3fvdfdo64ewa7rtebrq