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Construction of a Triple Gene-Deleted Live Vaccine Candidate against Pseudorabies Virus Using CRISPR/Cas9 and Cre/LoxP System-Based Strategy
Pakistan Veterinary Journal
The immune protective effects of pseudorabies virus (PRV) vaccines in the market have gradually reduced and they have failed to provide complete protection against the new PRV variant. In this study, a triple gene-deleted live PRV strain–rZDΔTK-gE-gI was successfully constructed by simultaneously knocking out the three major virulence genes (gE/gI and TK) with CRISPR/Cas9 and Cre/LoxP gene editing system and low melting point agarose purification method. After challenge with the virulent PRVdoi:10.29261/pakvetj/2021.053 fatcat:745bjevspbepvmp7uupvryji34