Co-Regulated Pendrin and Aquaporin 5 Expression and Trafficking in Type-B Intercalated Cells under Potassium Depletion

Giuseppe Procino, Serena Milano, Grazia Tamma, Silvia Dossena, Claudia Barbieri, Maria Celeste Nicoletti, Marianna Ranieri, Annarita Di Mise, Charity Nofziger, Maria Svelto, Markus Paulmichl, Giovanna Valenti
2013 Cellular Physiology and Biochemistry  
We recently reported that aquaporin 5 (AQP5), a water channel never identified in the kidney before, co-localizes with pendrin at the apical membrane of type-B intercalated cells in the kidney cortex. Since co-expression of AQP5 and pendrin in the apical membrane domain is a common feature of several other epithelia such as cochlear and bronchial epithelial cells, we evaluated here whether this strict membrane association may reflect a co-regulation of the two proteins. To investigate this
more » ... vestigate this possibility, we analyzed AQP5 and pendrin expression and trafficking in mice under chronic K + depletion, a condition that results in an increased ability of renal tubule to reabsorb bicarbonate, often leads to metabolic alkalosis and is known to strongly reduce pendrin expression. Methods: Mice were housed in metabolic cages and pair-fed with either a standard laboratory chow or a K + -deficient diet. AQP5 abundance was assessed by western blot in whole kidney homogenates and AQP5 and pendrin were localized by confocal microscopy in kidney sections from those mice. In addition, the short-term effect of changes in external pH on pendrin trafficking was evaluated by fluorescence resonance energy transfer (FRET) in MDCK cells, and the functional activity of pendrin was tested in the presence and absence of AQP5 in HEK 293 Phoenix cells. Results: Chronic K + depletion caused a strong reduction in pendrin and AQP5 expression. Moreover, both proteins shifted from the apical cell membrane to an intracellular compartment. An acute pH shift from 7.4 to 7.0 185 Procino et al.: Pendrin and AQP5 in Renal Cells Cellular Physiology and Biochemistry Cellular Physiology and Biochemistry caused pendrin internalization from the plasma membrane. Conversely, a pH shift from 7.4 to 7.8 caused a significant increase in the cell surface expression of pendrin. Finally, pendrin ion transport activity was not affected by co-expression with AQP5. Conclusions: The coregulation of pendrin and AQP5 membrane expression under chronic K + -deficiency indicates that these two molecules could cooperate as an osmosensor to rapidly detect and respond to alterations in luminal fluid osmolality.
doi:10.1159/000356638 pmid:24429825 fatcat:5jexya4a35abbcomqqp337ropu