Expression of a 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene in peach (Prunus persica L.) fruit in response to treatment with carbon dioxide and 1-methylcyclopropene: possible role of ethylene

M. Mathooko Francis, Tsunashima Yuki, Kubo Yasutaka, Inaba Akitsugu
2004 African Journal of Biotechnology  
In this study we investigated the effect of exogenous ethylene treatment on ethylene production, 1aminocyclopropane-1-carboxylate (ACC) oxidase activity and expression of an ACC oxidase (PP-ACO1) gene previously cloned (Mathooko et al., 2001) in peach (Prunus persica L.) fruit. We also investigated the mode of action of CO 2 and 1-methycyclopropene (1-MCP) in the regulation of ethylene biosynthesis during peach fruit ripening. Fruits were treated with various concentrations of ethylene (0.1, 1,
more » ... 10, 100, 500, 1000 ppm) and also with CO 2 and 1-MCP in the presence or absence of 500 ppm ethylene. Ethylene stimulated ethylene production at concentrations of 100 ppm and above while ACC oxidase activity was stimulated in a concentration-dependent manner. PP-ACO1 was slightly constitutively expressed and exogenous ethylene stimulated accumulation of its mRNA transcript in a concentration-dependent manner up to 100 ppm after which the level remained constant. CO 2 and 1-MCP inhibited the ethylenestimulated ethylene production, ACC oxidase activity and accumulation of PP-ACO1 transcripts by about 50%. These results indicate that ethylene plays a key role in the regulation of ethylene production and ACC oxidase activity and its gene expression in peach fruit. Further the results indicate that CO 2 and 1-MCP regulate ethylene biosynthesis in peach fruit during ripening, at least in part, by antagonizing ethylene action.
doi:10.5897/ajb2004.000-2098 fatcat:5kzsa3rhsvbnrd4rmwtw2w2uhy