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Transcriptome-wide sites of collided ribosomes reveal principles of translational pausing
Translation initiation is considered overall rate-limiting for protein biosynthesis, whereas the impact of non-uniform ribosomal elongation rates is largely unknown. Using a modified ribosome profiling protocol based on footprints from two closely packed ribosomes (disomes), we have mapped ribosomal collisions transcriptome-wide in mouse liver. We uncover that the stacking of an elongating onto a paused ribosome occurs frequently and scales with translation rate, trapping ~10% of translatingdoi:10.1101/710061 fatcat:e525gul745dsxg6unw5pjd7x6y