PHYSICAL AND IMMUNOBIOLOGICAL STUDIES OF TEICHOIC ACIDS OF PROBIOTIC LACTOBACILLI STRAINS

О. П. Лівінська, O. M. Василенко, T. В. Томила, I. Л. Гармашева, Н. К. Коваленко
2014 Mìkrobìologìâ ì Bìotehnologìâ  
Aim. The aim of this study was to establish the chemical structure of teichoic acids of probiotic lactobacilli strains and to investigate their influence on murine macrophages phagocytosis and proliferation of the murine macrophages cell line J 774. Methods. Physical (infrared spectroscopy methodology, 1 H, and 13 C NMR experiments), immunobiological and statistical methods were used. Results. It was shown that cell walls of Lactobacillus plantarum 11/16 and Lactobacillus plantarum 195D
more » ... d glycerol teichoic acids with unusually high number of sugar elements. The teichoic acid of the strain L. plantarum 11/16 had 9 different pyranoses and furanoses sugars in alfa-configuration and L. plantarum 195D had 4 hexosopyranoses. The teichoic acids of L. plantarum 195D and 11/16 increased the percent phagocytosis by 15.00±5.72% and 13.75±4.65% respectively. Both teichoic acids in the doses of 32 and 64 µg/ml stimulated cell proliferation of murine macrophage cell line by 35-53%. It was not observed any considerable effect of studied teichoic acids on phagocytic index, spontaneous and stimulated NBT-test of peritoneal macrophages of mice. K e y w o r d s : teichoic acids, NMR spectroscopy, infrared spectroscopy, phagocytosis, probiotics.
doi:10.18524/2307-4663.2014.4(28).48394 fatcat:b3udzfl5xneztkwbjhkciycha4