Silencing of the gene for homeobox protein HOXB13 by siRNA confers resistance to methylmercury on HEK293 cells

Gi-Wook Hwang, Katsunori Ryoke, Tsutomu Takahashi, Akira Naganuma
2010 Journal of Toxicological Sciences  
Methylmercury is an environmental pollutant that causes severe central nervous system disorders. We searched for transcription factors involved in the development of methylmercury toxicity and found that the decreased expression of a homeobox protein, HOXB13, in HEK293 cells conferred strong resistance to methylmercury. Fig. 2. Effects of HOXB13 knockdown on the sensitivity of HEK293 cells to various metal compounds. HEK293 cells (5 × 10 3 cells/well) that had been transfected with two kinds of
more » ... d with two kinds of HOXB13 siRNA were seeded in 96-well plates and cultured in 100µl aliquots of DMEM. For further details, see the legend to Fig. 1A. Vol. 35 No. 6
doi:10.2131/jts.35.941 pmid:21139345 fatcat:gqil3veduzh57mkxkfjfrmngou