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Molecular Modeling and Site-directed Mutagenesis Define the Catalytic Motif in Human γ-Glutamyl Hydrolase
2000
Journal of Biological Chemistry
Human ␥-glutamyl hydrolase (hGH) is a central enzyme in folyl and antifolylpoly-␥-glutamate metabolism, which functions by catalyzing the cleavage of the ␥-glutamyl chain of substrates. We previously reported that Cys-110 is essential for activity. Using the sequence of hGH as a query, alignment searches of protein data bases were made using the SSearch and TPROBE programs. Significant similarity was found between hGH and the glutamine amidotransferase type I domain of Escherichia coli
doi:10.1074/jbc.m007908200
pmid:11005824
fatcat:u32uqe3lwncenbgzr2t3y3454a