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A method using high-performance liquid chromatography has been developed and validated for determination of verbascoside in alcoholic crude extract of the aerial parts of B. stachyoides. Analysis was performed on a Phenomenex® Gemini-NX C18 analytical column (250 mm × 4.6 mm; 5 µm) using a mobile phase (pump A -aqueous solution containing H 2 SO 4 (0.01 M), H 3 PO 4 (0.4%), and (C 2 H 5 ) 2 NH (0.4%); pump Bmethanol:aqueous (95:5) solution containing H 2 SO 4 (0.05 M), H 3 PO 4 (2%), and (C 2 Hdoi:10.5935/0100-4042.20140057 fatcat:swgvaziqcragdlk2cqgr6wlgji