Isolation of verbascoside and validation of method to standardize the crude extract of the aerial parts ofBuddleja stachyoidesCham. & Schltdl. (Scrophulariaceae)

Daniella M. S. de Oliveira, Marilis D. Miguel, Milena Kalegari, Obdúlio G. Miguel, Thais F. Moreira
2014 Química Nova  
A method using high-performance liquid chromatography has been developed and validated for determination of verbascoside in alcoholic crude extract of the aerial parts of B. stachyoides. Analysis was performed on a Phenomenex® Gemini-NX C18 analytical column (250 mm × 4.6 mm; 5 µm) using a mobile phase (pump A -aqueous solution containing H 2 SO 4 (0.01 M), H 3 PO 4 (0.4%), and (C 2 H 5 ) 2 NH (0.4%); pump Bmethanol:aqueous (95:5) solution containing H 2 SO 4 (0.05 M), H 3 PO 4 (2%), and (C 2 H
more » ... 4 (2%), and (C 2 H 5 ) 2 NH (0.2%); pump C -acetonitrile:aqueous (90:10) solution containing H 2 SO 4 (0.05 M) and H 3 PO 4 (2%)) and a diode array detector at 325 nm. The method was validated in accordance with ANVISA guidelines and may be applied to quality control of herbal medicine with aerial parts of B. stachyoides.
doi:10.5935/0100-4042.20140057 fatcat:swgvaziqcragdlk2cqgr6wlgji