Passive transfer of immunity from Tilapia antisera enhanced in-vitro phagocytosis of Aeromonas hydrophila in naïve Macrobrachium rosenbergii haemocytes

C.M. Chong, Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia, A.S. Yaakob, A. Maha, Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
2020 Journal of environmental biology  
Aim : Methodology: Results : Interpretation : Key words: To determine the effectiveness of antisera derived from a teleost to augment the phagocytic activity of prawn phagocytes. Antisera from tilapia, Oreochromis niloticus, immunised with methanol-killed Aeromonas hydrophila was added to the suspension of A. hydrophila prior to the incubation with the haemocytes isolated from Macrobrachium rosenbergii and examined for phagocytosis. The sera derived from the sterile saline-injected fish were
more » ... jected fish were used for comparison. In vitro phagocytosis assay showed that the supplementation of immunised sera significantly enhanced Aeromonas uptake by prawn haemocyte by increasing phagocytic index to 3.23 ± 0.44, compared to control with phagocytic index of 1.35 ± 0.42. Tilapia antisera may potentially be adopted to provide passive immune-protection to crustaceans which lack adaptive immunity. Further studies should confirm the mechanisms involved in enhancement of prawn phagocytic uptake of bacteria by fish immunised sera.
doi:10.22438/jeb/41/5(si)/ms_05 fatcat:4x245vglkfc65idowfwfztdpnu