Two-dimensional crystallization of DNA gyrase B subunit on specifically designed lipid monolayers

L. Lebeau, E. Regnier, P. Schultz, J.C. Wang, C. Mioskowski, P. Oudet
1990 FEBS Letters  
The B subunit of DNA gyrase formed two-dimensional crystals when bound to a specIfically recognized phospholrpld spread mto a monolayer at the air/water interface. The especially deslgned hplds consisted of novoblocm coupled through the 3' or 2" hydroxyl group and a hydrophdous hnker of a given length to dloleoylphosphatldic acid. Two-dlmenslonal crystals of the gyrase B subumt are formed under physlologlcal condltlons of pH and lomc strength, with no precipitant added to the solution. Crystal
more » ... dfractlon extended to a 2 7 nm resolutmn m negative stam. with unit cell parameters a = 6 1 nm. h = 7.6 nm and 7 = 64 DNA gyrase, Novoblocin. Protem-lipld rnteractlon, Two-dImensIona crystalhzatlon; Electron m~roscopy: Image processmg Pubhshed by Elsevrer Scrence Publrshers B. V. (Blomedrcal Divrslon)
doi:10.1016/0014-5793(90)80282-n pmid:2163898 fatcat:qe6uizer4ndk3hu4mwfah7z4pi