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Auxotrophic mutants of the actinomycete Amycolatopsis methanolica requiring L-Phe or L-Tyr were isolated and identified as strains lacking prephenate dehydratase (strain GH71) or arogenate dehydrogenase (strain GH70), respectively. A. methanolica thus employs single pathways only for the biosynthesis of these aromatic amino acids. Anion-exchange chromatography of extracts revealed two peaks with Phe as well as Tyr aminotransferase (AT) activity (Phe/Tyr ATI and Phe/Tyr ATII) and three peaksdoi:10.1128/aem.61.4.1298-1302.1995 fatcat:5eucijjdbjcstpnobakghgxw5m