Characterisation of a Neutral Protease Produced by Micrococcus luteus

M.O. Ilori, O.O. Amund, O. Omidiji
1996 Zeitschrift für Naturforschung C - A Journal of Biosciences  
A proteolytic enzyme produced by a cassava-ferment­ing strain of Micrococcus luteus was extracted and puri­fied 50-fold by gel filtration and ion exchange chromatography. The optimum pH for the enzyme was 7.0, the optimum temperature 25 °C, the apparent molecular weight 42 kDa and the Km value, 0.45 mg ml-1 with casein as substrate. The enzyme was stimulated by Ca2+ and Mg2+ but inhibited by Zn2+ and Co2+ ions. Other inhibitors were EDTA, KCN, citric acid and L-cysteine indicating the enzyme to
more » ... ating the enzyme to be a metalloprotease.
doi:10.1515/znc-1996-5-623 fatcat:stn4hgvv5nfuvnyxi55sqlzope