Comparison of the Simple Cyclic Voltammetry (CV) and DPPH Assays for the Determination of Antioxidant Capacity of Active Principles

Jesús F. Arteaga, Mercedes Ruiz-Montoya, Alberto Palma, Gema Alonso-Garrido, Sara Pintado, José M. Rodríguez-Mellado
2012 Molecules  
Antioxidant activity of a number of small (low molecular weight) natural compounds found in spices, condiments or drugs (gallic acid, sesamol, eugenol, thymol, carvacrol, vanillin, salicylaldehyde, limonene, geraniol, 4-hexylresorcinol, etc.) has been evaluated using electrochemical and DPPH • radical scavenging measurements. Structural analysis of the tested compound suggest a remarkable activity for phenol derivatives and the importance of the -R groups located on the phenolic ring in the
more » ... lic ring in the molecule's ability to act as free radical scavenging as well as their influence in the electrochemical behavior. The voltammetric method can be used for the determination of the antioxidant capability in the same manner as the DPPH • radical scavenging because of the correlation found between oxidation potentials and anti-radical power (ARP = 1/EC 50 ). Such electrochemical determination is fast and cheap and allows making measurements under a variety of experimental conditions. The accuracy of the electrochemical measurements is the same for all the compounds, irrespective of their scavenging activity, the opposite of what occurs in the DPPH • test.
doi:10.3390/molecules17055126 pmid:22555300 fatcat:vlvpkx344vcmvoka5jg42bes6y