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We report the first record of Trachycephalus typhonius for the state of Rio Grande do Sul, Brazil, based on individuals found in the municipalities of Roque Gonzales and Salvador das Missões. The original vegetation of these municipalities is characterized as Mesophytic Semideciduous Forest (Atlantic Domain), currently replaced by agricultural activities and urbanization. This record expands the geographical distribution area of this species by approximately 270 km from the nearest known locality, Iguazú, Misiones province, Argentina.doi:10.15560/8.4.817 fatcat:jkemd3kjhbc3nhvs26ulzjh6se