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Hypoxia and autophagy are known to facilitate tumor progression. We here aimed to investigate the role of hypoxia-associated autophagy in cholangiocarcinoma (CCA) survival and metastasis. Immunostaining of hypoxicresponsive proteins (HIF-1α and BNIP3) and a key regulator of autophagy (PI3KC3) were examined in CCA tissues and their expression levels were compared with clinicopathological parameters. A hypoxia mimicking condition (CoCl 2 treatment) was also tested regarding CCA cell functions.doi:10.7314/apjcp.2014.15.14.5873 fatcat:cdx5c5tt4bhl5lfzrcc662fr64