Physiobiochemical and microbiologic stability characteristics of freeze-dried cartilage secretome Adipose Mesenchymal Stem Cell (AdMSC)

Ferdiansyah Danang Perwira, Dwikora Novembri Utomo, Lukas Widhiyanto, Kukuh Dwiputra Hernugrahanto
2022 International Journal of Health Sciences  
Cartilage is an avascular, alymphatic, and aneurysmal structure, so it is difficult to heal properly if an injury occurs. Using Chondrogenic Adipose Mesenchymal Stem Cell (AdMSC) secretome as an adjuvant is one of the therapeutic options to overcome the problem of injury to the cartilage. Storage and distribution problems must be solved if we use this method. This study aims to characterize the Freeze-dried chondrogenic AdMSC secretome. Secretome's physical, biochemistry, and microbiology
more » ... ity are derived from the chondrogenic AdMSC secretome. This study's design is prospective observational analytics research. Freeze-dried cartilage secretome AdMSC was analyzed for physical (organoleptic changes (shape, color, and odor), pH, biochemistry (TGF-β3), and microbiology (microbe contamination) stability. The difference was observed in 0, 4, 8, and 12 weeks. Data normality and homogeneity were tested using the Shapiro-Wilk test and followed up with the Friedman and Wilcoxon tests to analyze the data further. Organoleptics change showed no color change until four weeks with no change of shape and smell until 12 weeks. No significant difference was found in porosity and pH change—TGF-β3, which has a role in chondrogenesis, has no considerable decrease until 12 weeks. No contamination was found until 12 weeks.
doi:10.53730/ijhs.v6ns5.11641 fatcat:vhjyx3r3f5gxth2famw6m6ghbi