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Aim: Describe levels of depression, anxiety and stress among users of an urban/rural health centre, analyse sex differences and comorbidity between depression, anxiety and stress. Method: Descriptive co-relational study with a Portuguese version of DASS-21; consecutive sample (n=343). Results: 40.52%, 43.48% and 45.06% of individuals present some degree of depression, anxiety and of stress, respectively. Severe or very severe levels of disturbance were found in 20.87% (anxiety), 22.38% (stress)doi:10.1590/s0104-11692011000200017 pmid:21584382 fatcat:mjugpy45pna6flf7zvdtr4uwb4