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Chitosan microspheres with a mean size of 140 ± 119 µm were produced by the spray and coagulation methods. The microspheres were chemically modified using the following routes: a) crosslinking with glutaraldehyde b) crosslinking with epychlorohydrin and c) acetylation. For investigation of their ability as adsorbents, the following standard proteins were chosen as adsorbates: bovine serum albumin -BSA (pI = 4.8 and MW = 66 kDa) and lysozyme (pI = 11 and MW = 14 kDa). The adsorption experimentsdoi:10.1590/s0104-66322007000300003 fatcat:cgikemk3nva4zanwwwufipuowq