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Developed a thin layer chromatography (TLC) method for the quantification of piperine in Trikatu Churna formulation it is a major and important ingredient in the formulation. TLC methods for the determination of piperine in the Trikatu churna along with its raw materials have been developed, as per the ICH guideline. The developed method has been validated, experimented with parameters like linearity, accuracy, the limit of detection, the limit of quantification, inter-day and intra-day assaydoi:10.9734/ajocs/2020/v8i419054 fatcat:7mnnv7cqjzdpvmxxdgozqujk7a