Regeneration of Plantlets from Rhizome Bud Explants of Lasia spinosa (Lour.) Thwaites- A Medicinal Plants of Assam

Puspita Hore, Bhaben Tanti
2018 International Journal of Life-Sciences Scientific Research  
ABASTRACT In numerable medicinal plants are commercially propagated through tissue culture for large production of elite material (Rhizome buds of Lasia spinosa {Lour.}). Thwaites could be induced on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of kinetin (kin) and 6-benzyl amino purine (BAP) alone and in combination with Naphthaleneacetic acid (NAA). Lasia is one of the traditionally important plants of Assam, which is employed in the treatment of
more » ... gastrointestinal diseases, respiratory diseases and skin infections. This plant is also a rich source of dietary fibers, reported containing polyphenols, ascorbic acid and hydrocyanic acid. The present study aimed to establish producible protocol for in vitro regeneration of Lasia spinosa using rhizome bud explants. For shoot proliferation, among the various concentrations, 3.0 mgL -1 BAP showed the highest shoot regeneration frequency of 88.2±2.8%. The highest number of shoot were recorded as 1.9 ± 0.45 in L. spinosa, but the highest shoot length (4.5±0.07 cm) was observed at reduced concentration of BAP (1.0 mgL -1 ). Plantlets rooted in ½ strength MS medium augmented with 0.1-1.0 mgL -1 either NAA or IBA for L. spinosa for root formation. The highest percentage (79.5±2.6%), maximum number of rootlets/ shoot let (4.0±0.46) and mean length of rootlets (3.25±0.06 cm) were observed in L. spinosa. Our findings have paved a way for future investigation on another mode of regeneration like haploid production, anther culture etc and also for the commercial and rapid propagation of L. spinosa. The genetic diversity of medicinal plants in the world is getting endangered at an alarming rate because of ruinous harvesting practices and over-harvesting for production of medicines. As conventional propagation method through rhizome axillary buds is time consuming and provides a limited number of propagules, it is necessary to promote rapid production of L. spinosa through tissue culture techniques for its commercial availability and conservation. MATERIALS AND METHODS Explant sterilization-Excised micro-cutting of rhizome bud from the source plants were used as explants. The explants were coarsely trimmed to a size of 3 cm and washed thoroughly under running tap water for 10 min and then treated with liquid detergent [5% (v/v) Tween-20] for 15 min. Later these explants were washed with double-distilled water for 10 min. The explants were then sterilized with 0.1% (w/v) mercuric chloride (HgCl2) for 5 min and washed several times with sterile H2O to remove all traces of HgCl2. After a final wash, the Research Article
doi:10.21276/ijlssr.2018.4.3.1 fatcat:gnmaqn46obccfki6hxhk5pi3dm