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Little more than a quarter of a century has elapsed since Yersin and Kitasato independently described the bacterium of bubonic plague in 1893. Within four years thereafter, Haffkine first reported the possibility of protective vaccination against the disease caused by B. pestis. The announcement was followed by a number of investigations designed to test the type of vaccine most suitable for the purpose. Haffkine's procedure involved the use of killed broth cultures of the micro-doi:10.1001/jama.1921.02630270040017 fatcat:b427zbfcyvcvrdt4zagf7g7fgy