THE FATE OF INJECTED 4:4' DIAMINODIPHENYL-SULPHONE IN HUMANS AND GUINEA PIGS

I. A. SIMPSON, B. D. MOLESWORTH
1950 Leprosy Review  
During clinical trial s oJ the value of 4A' diaminodiphenyl s ulphon e ll1 the treatment of leprosy (r.10Iesworth and Narayanaswami (I)), a considerable number of estimations have been made to determine blood sulphone levels. The method, which has been used, depends on the development of a purplish red col ou r by coupling the diazotised s u l p hone with N (I-Naphthyl )-ethylene diamine and has proved satisfa ctory for l::!ood . At th e same time, it was considered to be desirable to esti m a
more » ... irable to esti m a te tissue and urine concentrations and an adaptation of the method emp loyed [or b lood was tried for this purpose, and is described in detail below. It was , however, found that, although the colour, developed from extracts of venous blood which con tained s ulphone , was similar to that dev e l oped by standard aqueous solutions of the pure sulphone-namely a purplish red the colour developed by extracts of tissues and by urine, after clarification with trichloracetic acid differed appreciably by being considerab ly more pink in shade . It was considered that this observation w arranted further study which is now described. Some values of s u lp hone levels found in tissues of guinea pigs that had received a series of inj ections of sulphone in oil and in tissues obtained post-mortem from a few human cases, that had been undergoing su lphone treatment, arc also recorded.
doi:10.5935/0305-7518.19500002 fatcat:a7luoucijraqdgv67oohjyiuoq