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Original Article Establishment and characterization of an immortalized rat hepatic stellate cell line
Int J Clin Exp Pathol
Hepatic stellate cells (HSCs) play an important role in liver fibrosis and portal hypertension. This study established a new rat HSC cell line LSC-1. Liver ex vivo perfusion with collagenase IV and density gradient centrifuga-tion were used to isolate rat HSC. Cells have been maintained in culture for multiple passages. LSC-1 cell biological characteristics were studied. LSC-1 cell have been maintained in culture over 100 passages. This new HSC cell line express telomerase reverse transcriptasefatcat:tmex6h7ilfa7boirymofpooklu