Osteoporosis is a common disease characterised by a systemic impairment of bone mass and microarchitecture that results in fragility fractures. With an ageing population, the medical impact of osteoporosis will increase further. A detailed knowledge of bone biology with molecular insights into the communication between bone-forming osteoblasts and bone-resorbing osteoclasts and the orchestrating signalling network has led to the identification of novel therapeutic targets. Based on this, novel

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... reatment strategies have been developed aimed at inhibiting excessive bone resorption and increasing bone formation. The most promising novel treatments include denosumab, a monoclonal antibody against receptor activator of NF-κB ligand, a key osteoclast cytokine, odanacatib, a specific inhibitor of the osteoclast protease cathepsin K, and antibodies against the sclerostin, an endogenous inhibitor of bone formation. In the past decade, the pathogenesis of osteoporosis has been linked to processes at the tissue, cellular, and molecular levels. Signals that act as "master-switches" have been defined that integrate various endocrine, neuroendocrine, inflammatory, and mechanical stimuli. At the cellular level, communication and coupling between the main bone cell types, the bone-forming osteoblasts and the bone-degrading osteoclasts constitute the smallest functional unit. Several key molecules conduct the coordinated activities of osteoblasts and osteoclasts during bone remodeling. Detailed knowledge of the molecular and cellular players has created a new concept of bone pathophysiology. With some of these new principles finding their way into clinical practice, we will highlight the most relevant advances in the field. Osteoclasts originate from haematopoietic stem cells and are closely related to monocytes and macrophages. Differentiation from osteoclast precursor cells to fully activated multinucleated osteoclasts depends critically on the presence of receptor activator of NF-κB ligand (RANKL), a member of the TNF family, and the permissive role of macrophage colony-stimulating factor (M-CSF). Cathepsin K represents a key cystein proteinase of the mature osteoclast that degrades collagen and breaks down bone. Cathepsin K is a critical determinant of resorptive activity by osteoclasts which removes bone of poor quality, where micro-cracks have accumulated and leaves hole-like lacunae. The osteoblast represents a unique bone-forming cell derived from mesenchymal stem cells. The speed and efficacy of precursor cells differentiating into mature osteoblasts that secrete matrix that can be mineralised and their life span determines the rate of bone formation. At the molecular level, activation of the canonical Wnt/β-catenin pathway is the master switch for osteoblastic differentiation. This key bone-anabolic pathway is negatively regulated by Wnt inhibitors such as dickkopf-1 (Dkk-1) and sclerostin which bind and block the Wnt receptor LRP-5. Denosumab The prominent role of RANKL in osteoclastogenesis has made it a prime target for therapy against diseases characterised by excessive bone loss. This led to the development of denosumab, a fully human monoclonal antibody against RANKL. Denosumab displays a high specificity and affinity for RANKL, translating into a longer dosing interval of 6 month. A large study program on a wide spectrum of bone diseases, including several types of osteoporosis and bone metastases is currently ongoing. With completed phase 3 studies, denosumab is the most advanced of all investigational substances and has 2010 been approved in Europe and the US for the treatment of osteoporosis. In the phase 1 study, a single subcutaneous injection dosedependently suppressed urinary N-terminal telopeptide of type I collagen (NTX), a biochemical marker of bone resorption, by up to 81% in postmenopausal women for as long as 6 months and was well-tolerated. Subsequently, a phase 2 study with different doses (6 -210 mg) and intervals (every 3 to 6 months) was aimed at evaluating the effects on BMD after 12 months in postmenopausal women with low bone mass. A dose-dependent suppression of bone turnover was observed, with a decrease of the bone resorption marker serum CTX as early as three days after administration and a maximum reduction of 88 % in the denosumab groups. Of note, discontinuation of denosumab treatment caused a rapid increase of bone turnover markers to values above baseline and even greater than those observed in the placebo group. Long term follow-up is required to determine the clinical effects of suppressed bone turnover during denosumab therapy. In a pivotal randomised placebo-controlled phase 3 study (FREEDOM), denosumab was assessed for its fracture reduction in 7,868 women with postmenopausal osteoporosis, of whom 24 % had preexisting vertebral fractures. After 3 years, denosumab had reduced the risk of new radiographic vertebral fractures by 68 %, hip fractures by 40 %, and non-vertebral fractures by 20 %. Of note, the risk of cardiovascular events, cancer, and infections did not differ between the two groups.