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Cell-penetrating Peptides
2002
Journal of Biological Chemistry
Cellular uptake of a family of cationic cell-penetrating peptides (examples include Tat peptides and penetratin) have been ascribed in the literature to a mechanism that does not involve endocytosis. In this work we reevaluate the mechanisms of cellular uptake of Tat 48 -60 and (Arg) 9 . We demonstrate here that cell fixation, even in mild conditions, leads to the artifactual uptake of these peptides. Moreover, we show that flow cytometry analysis cannot be used validly to evaluate cellular
doi:10.1074/jbc.m209548200
pmid:12411431
fatcat:rhkiad2gmvacxcslmkfbnnceim