Fungicidal and bactericidal activity of alkyl-substituting polyetherguanidines

M. Ya. Vortman, Institute of Macromolecular Chemistry, NAS of Ukraine, Yu. B. Pysmenna, A. V. Rudenko, V. V. Tretyak, V. N. Lemeshko, V. V. Shevchenko, D. K. Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, Institute of Urology of the National Academy of Medical Sciences of Ukraine, Institute of Urology of the National Academy of Medical Sciences of Ukraine, Institute of Macromolecular Chemistry, NAS of Ukraine, Institute of Macromolecular Chemistry, NAS of Ukraine
2020 Біологічні студії  
Vortman M. Ya., Pysmenna Yu. B., Rudenko A. V., Tretyak V. V., Lemeshko V. N., Shevchenko V. V. Fungicidal and bactericidal activity of alkyl-substituting polyetherguanidines. Studia Biolo gica, 2020: 14(3); 65-78 • DOI: https://doi.org/10.30970/sbi.1403.630 Results. The synthesis of polyetherguanidinium iodide is carried out in four stages. The first stage is the synthesis of a guanidine-containing oligoether with terminal guanidine moieties by the reaction between aromatic oligoepoxide and
more » ... oligoepoxide and guanidine. The second stage is the synthesis of polyetherguanidinium chloride by the reaction between guanidinium-containing oligoether with terminal guanidine fragments and oligooxyethylenediamine of different molecular weight. In the third stage, the obtained polyetherguanidinium chloride is converted from the salt form to the base form by the reaction with an equivalent amount of alkali in ethanol. In the fourth stage, the basic polyetherguanidine is reacted with methyl iodide at a molar ratio of polyetherguanidine: methyl iodide components of 1:2. The bactericidal and fungicidal activities of alkyl-containing polyetherguanidinium iodides against various heterotrophic bacteria and microscopic fungi has been shown. It was found that polyetherguanidinium iodides at a concentration of 1-3% inhibited the growth of Gram-negative (Escherichia coli 475, Klebsiella pneumonia 479) and Gram-positive (Staphylococcus aureus 451) bacteria. The obtained alkyl-containing polyetherguanidinium iodides at a concentration of 1% for 14 days showed fungicidal activity in almost all studied isolates. With an increasing length of the oligoethylene oxide component, the obtained polymers with n = 10 and 50 did not show a fungicidal effect against Aspergillus niger, and the polymer with n = 50 -against the micromycete Aspergillus versicolor. If we determine the fungicidal effect as a whole, the polymer with n = 6 had the highest activity against all studied isolates after 14 days, while the fungicidal effect of polymers with n = 10 and n = 50 tends to decrease. All synthesized alkylcontaining polyetherguanidinium iodides showed the highest activity against such microscopic fungi as Cladosporium cladosporioides, Acremonium strictum, Alternaria alternate, Cladosporium sphaerospermum, Paecilomyces variotii, Stachybotrys chartarum. Also noteworthy is the selective effect of the obtained polymers on individual isolates. Conclusions. The obtained polyetherguanidinium iodides of different molecular weight at a concentration of 1-3% showed bactericidal activity against Staphylococcus aureus 451, Escherichia coli 475, Klebsiella pneumoniae 479 and fungicidal effect against all fungi studied by us and can be used as disinfectants for indoor treatment.
doi:10.30970/sbi.1403.630 fatcat:revc3xyjofdqxp45fpvr7dftbu