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The present study tests the effect of different elicitor on cell biomass and alkaloid production in Eurycoma longifolia cells. The cell suspension cultures were prepared by inoculating 0,5g FW (fresh weight) of cells obtained from first batch of suspension cultures prepared from callus in liquid MSBs medium. Different concentration of chitosan, NaH2PO4, Na2CO3 and polyvinylpyrrolidone (PVP) were added as elicitor to optimize cell biomass and alkaloid production. MSBs medium supplementeddoi:10.22458/urj.v2i2.160 fatcat:52zk2va36nctzbc3hub37stude