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To utilize starch and protein contained in microalgae as carbon and nitrogen sources for ethanol production, an extraction method, i.e. ultrasonic treatment using a homogenizer, and simultaneous saccharification and fermentation (SSF) of extracted microalgae solution were studied using Chlamydomonas fasciata Ettl 437. 30 min of ultrasonic treatment gave the maximum extraction ratio of starch contained in microalgae, i.e. 93.8%, that corresponded to 0.408 g-starch/g-dry microalgae. SSF of thedoi:10.4236/nr.2012.34023 fatcat:7luu7gkhr5bj7lg5icq2v24iiy