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In vitro Cytological Studies of Leaf Callus Cultures of Orthosiphon aristatus (Blume) Miq
British Biotechnology Journal
The study was carried out to unravel the in vitro cytological behaviour of leaf callus cultures of Orthosiphon ariststus (Blume) Miq. The cultures were raised using leaf explants on Murashige and Skoog (MS) medium supplemented with different growth regulators like auxins (2,4-D, IAA, NAA) and cytokinin (BAP) alone and in different concentrations and combinations. Best callusing from the leaf explants was achieved in 2,4-D supplemented MS medium at 5 mg/l concentration. Cytogenetic analysis ofdoi:10.9734/bbj/2016/25638 fatcat:jhhhgsr6ubgrjlzvwxvwvnaeji