Seletividade de Beauveria bassiana e Metarhizium anisopliae a Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae)
Selectivity of Beauveria bassiana and Metarhizium anisopliae to Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) ABSTRACT -Trichogramma pretiosum Riley and the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae are effi cient biological control agents and are thought to be used jointly. In here, we investigated if these entomopathogens could have any side-effects on T. pretiosum. Therefore, 1 x 8 cards containing sterilized eggs of Anagasta kuehniella (Zeller)
... niella (Zeller) that were sprayed with 0.2 ml of B. bassiana or M. anisopliae (1.0 × 10 9 conidia/ml) were offered to a T. pretiosum female for 24h (30 cards/fungus = 30 replicates). Afterwards, females were isolated in glass tubes. The control group was sprayed with sterile distillated water + Tween 80 (0.01%). In addition, 60 cards with sterilized eggs of A. kuehniella were submitted to parasitism by females of T. pretiosum for 24h. Of these cards, 30 were sprayed with B. bassiana or M. anisopliae and 30 with distillated water + Tween 80 (0.01%), and observed daily until parasitoid emergence. Metarhizium anisopliae decreased parasitoid emergence and caused confi rmed mortality. Therefore, fi eld and semi-fi eld experiments should be conducted for a fi nal assessment of the side-effects of these entomopathogens on Trichogramma as a ways to develop a control strategy in which both can be used.